目的建立应用电感耦合等离子体质谱法(ICP-MS)测定穿心莲药材中19种微量元素包括钠、镁、铝、硅、钾、钛、铬、锰、铁、钴、镍、铜、锌、砷、银、镉、锡、汞、铅含量的测定方法。方法样品经微波消化后直接采用八极杆碰撞/反应池技术结合电感耦合等离子体质谱法(ICP-MS)进行测定。结果测定方法中所有元素的线性相关系数(r)都大于0.9990，检出限在0.001～0.051μg·kg-1之间，重复性和稳定性的相对标准偏差(RSD)都小于10%，药材的加标回收率在88.64%～102.98%之间，符合痕量分析的要求。结论本方法检出限低、操作简单、快速，且灵敏度高，可作为穿心莲药材中微量元素的定量分析。
Abstract
OBJECTIVE To establish an ICP-MS method for simultaneous determination of 19 trace elements,i.e. Na,Mg,Al,Si,K,Ti,Cr,Mn,Fe,Co,Ni,Cu,Zn,As,Ag,Cd,Sn,Hg,and Pb,in Andrographis Herba. METHODS After microwave digestion treatment,samples were directly determined by inductively coupled plasma mass spectrometry based in octopole reaction system (ORS). RESULTS The validated method indicated that the correlative coefficients (r) for all elements were above 0.999 0. The limits of detections were in the range of 0.001-0.051 μg·kg-1. The reproducibility and stability were satisfactory with all RSDs below 10%. The spiked recoveries for Andrographis paniculata were between 88.64% and 102.98%. CONCLUSION The method is simple,rapid and sensitive,which meets the requirement of trace analysis and can be used for the quality control of Andrographis Herba.
关键词
穿心莲 /
微量元素 /
电感耦合等离子体质谱法 /
八级杆碰撞/反应池
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Key words
Andrographis paniculata /
trace element /
ICP-MS /
octopole collision/reaction system(ORS)
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中图分类号：
R917
R284
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参考文献
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脚注
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基金
十二五国家科技支撑计划课题(编号2011BAI01B01)
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